Abstract: Objective To establish three genotyping methods for immunodeficient mice. Method According to the genotype identification sequences of three immunodeficient mice, B6. Cg-Foxn1 nu / J, B6. Cg-Prkdc scid / SzJ, and C57BL / 6J-Lyst bg-J / J, provided on The Jackson Laboratory website, primers were designed and PCR reaction programs were optimized to establish PCR detection methods for corresponding spontaneously mutated genes. I n addition, the above corresponding methods were applied to genotype identification of BALB / cAnSlacNifdc-nu, SCID / Nifdc, and C57BL / 6JNifdc-bg mice preserved in the National Rodent Laboratory Animal Resources Center. Result Three simple methods were established using PCR combined with Sanger sequencing to detect spontaneous mutations in Foxn1 nu , Prkdc scid , and Lyst bg-J genes. Furthermore, the corresponding method were used to detect mutation sites in the genomes of three inbred mice strains, including BALB / cAnSlacNifdc-nu, SCID / Nifdc, and C57BL / 6JNifdc-bg, which were preserved in the National Rodent Laboratory Animal Resources Center. The genotyping results of the three mice strains were consistent with the corresponding information published on The Jackson Laboratory website. Conclusion The simple method for genotype identification of immunodeficient mice established in this study are beneficial for controlling and monitoring the genetic quality of immunodeficient mice. 

Key words: immunodeficient mice, genetic quality, genotype identification, PCR detection methods, spontaneously mutated genes

摘要： 目的 建立适用于 3 种免疫缺陷小鼠基因型的鉴定方法。 方法 参考 The Jackson Laboratory 网站中提供的 B6. Cg-Foxn1 ^nu / J、B6. Cg-Prkdc ^scid / SzJ 和 C57BL / 6J-Lyst ^bg-J / J 等 3 种免疫缺陷小鼠的基因型鉴定序列进行设计引物 和 PCR 反应程序优化,从而建立了相应自发突变基因的 PCR 检测方法。 应用上述相应的方法对国家啮齿类实验 动物资源库保存的 BALB / cAnSlacNifdc-nu、SCID / Nifdc 和 C57BL / 6JNifdc-bg 等 3 个品系的小鼠进行了基因型鉴定。 结果 采用 PCR 检测方法结合 Sanger 测序建立了可以检测 Foxn1 ^nu 、Prkdc ^scid 和 Lyst ^bg-J 基因自发突变的简易方法, 同时使用建立的相应方法检测国家啮齿类实验动物资源库中保存的 BALB / cAnSlacNifdc-nu、SCID / Nifdc 和 C57BL / 6JNifdc-bg 等 3 种近交系小鼠基因组中突变位点与 The Jackson Laboratory 网站中公布的信息相吻合。 结论 本研 究中建立了 3 种免疫缺陷小鼠的基因型鉴定的简易方法,有利于对免疫缺陷小鼠遗传质量的控制与监测。

关键词: 免疫缺陷小鼠, 遗传质量, 基因型鉴定, PCR 检测方法, 自发突变基因